By S. Handy

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2006). 5) from 0 to 5% (v/v) (Fig. 2). When the concentration of the aqueous solution was 1% or lower, the production of methyl caffeate increased. However, the production of methyl caffeate was decreased in aqueous solutions greater than 2%. The production of caffeic acid was increased with addition of the buffer, indicating that the enzyme probably catalyzed hydrolysis of 5-caffeoylquinic acid to produce caffeic acid rather than alcoholysis to produce methyl caffeate. Thus, the result indicated that the addition of 1% aqueous solution was suitable for the production of methyl caffeate.

Oxidative Pretreatment of Pine Wood to Facilitate Delignification during Kraft Pulping. Holzforschung 1982, 36 (3), 123-130. [21] Hatakka, A. , Pretreatment of wheat straw by white-rot fungi for enzymic saccharification of cellulose. Applied Microbiology and Biotechnology 1983, 18 (6), 350357. ; Ragauskas, A. , Ionic Liquid as a Green Solvent for Lignin. Journal of Wood Chemistry and Technology 2007, 27 (1), 23-33. [23] Tan, S. S. ; MacFarlane, D. ; Edye, L. ; Doherty, W. O. ; Patti, A. ; Pringle, J.

When an IL, 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([bmim][NTf2]) was used as a reaction solvent, we found that immobilized chlorogenate hydrolase (Kikkoman) catalyzed the conversion of 5caffeoylquinic acid to methyl caffeate with methanol (Fig. 1). , 2011). To synthesize valuable compounds from caffeoylquinic acids, we attempted to develop a method for the conversion of caffeoylquinic acids to CAPE analogues via methyl caffeate. In section 2, we describe the properties of immobilised chlorogenate hydrolase in ILs.

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